Eimeria-Stimulated Intestinal IELs in Chickens
نویسندگان
چکیده
Intraepithelial lymphocytes (IELs) play a critical role in protective immune response to intestinal pathogens such as Eimeria, the etiologic agent of avian coccidiosis. A list of genes expressed by intestinal IELs of Eimeria-infected chickens was compiled using the expressed sequence tag (EST) strategy. The 14,409 ESTs consisted of 1851 clusters and 7595 singletons, which revealed 9446 unique genes in the data set. Comparison of the sequence data with chicken DNA sequences in GenBank identified 125 novel clones. This EST library will provide a valuable resource for profiling global gene expression in normal and pathogen-infected chickens and identifying additional unique immune-related genes. Index Entries: Chickens; intestinal lymphocytes; expressed sequence tag (EST); expression cDNA li-
منابع مشابه
Mucosal immunity against Eimeria acervulina infection in broiler chickens following oral immunization with profilin in Montanide™ adjuvants.
The present study was conducted to compare aqueous nanoparticle-based Montanide™ IMS 1313 N VG PR (IMS 1313) and oil-based ISA 71 VG (ISA71) adjuvants in combination with an Eimeria subunit protein vaccine on protection against avian coccidiosis. Male broiler chicks were vaccinated twice with an Eimeria recombinant profilin protein alone or in conjunction with IMS 1313 or ISA 71 prior to infect...
متن کاملChanges in immune-related gene expression and intestinal lymphocyte subpopulations following Eimeria maxima infection of chickens.
Coccidiosis, a major intestinal parasitic disease of poultry, induces a cell-mediated immune response against the etiologic agent of the disease, Eimeria. In the current study, the expression levels of gene transcripts encoding pro-inflammatory, Th1, and Th2 cytokines, as well as chemokines were measured in intestinal intraepithelial lymphocytes (IELs) after Eimeria maxima infection. In additio...
متن کاملNitric Oxide Production by Macrophages Stimulated with Coccidia Sporozoites, Lipopolysaccharide, or Interferon-c, and Its Dynamic Changes in SC and TK Strains of Chickens Infected with Eimeria tenella
Nitric oxide (NO) is an important mediator of innate and acquired immunities. In the studies reported here, we quantified NO produced in vitro by chicken leukocytes and macrophages and in vivo during the course of experimental infection with Eimeria, the causative agent of avian coccidiosis, and identified macrophages as the primary source of inducible NO. Eimeria tenella–infected chickens prod...
متن کاملMolecular cloning and characterization of chicken NK-lysin.
NK-lysin is an anti-microbial and anti-tumor protein expressed by NK cells and T lymphocytes. In a previous report, we identified a set of overlapping expressed sequence tags constituting a contiguous sequence (contig 171) homologous to mammalian NK-lysins. In the current report, a cDNA encoding NK-lysin was isolated from a library prepared from chicken intestinal intraepithelial lymphocytes (I...
متن کاملBacterial Population Changes in the Ceca of Young Chickens Infected with Eimeria tenella.
Eimeria tenella, a protozoan parasite belonging to the class Sporozoa, is the etiological agent of cecal coccidiosis of chickens. This organism goes through a typical sporozoan life cycle and, with the exception ofsporulation of the oocyst, all stages occur within the host's intestinal tract. Invasion of the intestinal epithelium by sporozoites and merozoites results in considerable tissue dest...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2005